The principle of blood glucose test paper in blood glucose meter?

1, the principle of blood glucose test paper in blood glucose meter is similar to that of urine analyzer.

2. Determination of glucose: Enzymatic method is used to determine glucose by test paper method. The membrane block contains glucose oxidase, peroxidase and chromogen. There are two main color sources used by different manufacturers: ① potassium iodide is the color source, and the positive reaction is red; ② Using o-toluidine as chromogenic reagent, the positive reaction was blue. The original amount is that glucose oxidase oxidizes glucose into glucuronic acid and hydrogen peroxide, and the latter is released by peroxidase to make chromogen develop color. This method is the most commonly used.

3. As far as the instrument structure itself is concerned, the blood glucose meter can be divided into electrochemical test and light reflection test according to the sugar measurement technology.

1) the electrochemical method test is that the electrons generated by the reaction between enzyme and glucose are read by current counting facilities and then converted into glucose concentration readings. Its advantage is relatively cheap, but its disadvantage is that the photoelectric blood glucose meter is similar to a CD machine, and the probe is exposed to the air, which is easy to be polluted, which affects the test results and has a large error. Generally, it is more accurate in a short time. After a period of use, it must be calibrated at the maintenance station.

2) light reflection technology: it is an intermediate (colored substance) produced by the reaction between enzyme and glucose. The detector detects the intensity of reflected light on the reflective surface of the test paper and converts the intensity of these reflected light into glucose concentration. This blood glucose meter is hidden in the electrode mouth, which can avoid pollution and has high accuracy.

4. The blood glucose meter needs to be calibrated regularly and can only be measured roughly. It should be based on the biochemical test in the hospital.

5. The determination of blood glucose meter is affected by current and voltage, bulb, test paper quality and soaking time.