How to disinfect explants of tissue culture seedlings?

After explants are obtained, they must be strictly sterilized, because explants are often accompanied by a variety of microorganisms, and once these microorganisms are brought into the culture medium, they will multiply rapidly, which makes tissue culture wasted. While killing or removing all kinds of microorganisms, the vitality of explants must be maintained. Rinse/kloc-0 with running tap water for 0/0 ~ 20 minutes, wash the dirty explants with washing powder, or lightly brush with a brush or writing brush to remove dirt, and then use filter paper to absorb the surface moisture of the explants after rinsing. Soak in 70% alcohol 15 ~ 30 seconds, and rinse with sterile water twice. Then disinfect with appropriate chemicals, such as sodium hypochlorite, hydrogen peroxide, bleaching powder, low concentration mercury chloride, etc. The use of these disinfectants can play a role in surface disinfection. The specific concentration and soaking time depend on the kind of explants and drugs. For example, soak in 2% ~ 10% sodium hypochlorite aqueous solution for 3 ~ 15 minutes. Rinse with sterile water for 3 ~ 5 times immediately after sterilization, wash off the liquid medicine, and absorb water with sterile filter paper to prepare for separation.