Advantages and disadvantages of determining pollen viability by magenta acetic acid staining method

Advantages and disadvantages of determining pollen viability by magenta acetic acid staining;

1, advantages: fuchsin acetate is often used as a fixing and staining agent for nuclei and chromosomes. When observing plant cell mitosis and staining chromosomes, it is necessary to use basic dye solution, and magenta acetate can be used.

2. Disadvantages: The measured value of magenta acetate method is too high to reflect the fertility and energy of pollen itself. The reference value is not great.