How is the nutritious Grifola frondosa made?

1. The selenium-enriched culture of Grifola frondosa is one of the essential trace elements for human body and an indispensable component of glutathione peroxidase. It has the function of protecting biofilm from lipid peroxide and free radicals. Selenium has the peculiar effects of cancer prevention, anticancer and anti-aging. Selenium deficiency is also closely related to vascular diseases such as coronary heart disease, arteriosclerosis and hypertension. If the anti-cancer effect of Grifola frondosa is combined with organic selenium to form organic selenium polysaccharide and selenium protein, it will be more beneficial to play the role of disease resistance. According to Xu and Xu, Grifola frondosa Gruv05 has a strong tolerance to selenium. When the concentration of selenium in solid medium is 300mg/kg (calculated as Se), mycelium can still grow. When the selenium concentration is 10~ 100mg/kg, selenium can obviously promote the growth of Grifola frondosa mycelium. When the selenium concentration is 10~ 100mg/kg, the total amount of mycelium enriched in liquid culture is between 6 1.4~882. 1mg/kg; When the selenium concentration is 50mg/kg, the absorption and transformation rate of mycelium is as high as 4.9%. When the concentration of selenium is 50mg/kg, the dry weight of mycelium can be increased and the utilization of reducing sugar in culture medium can be promoted. The concentration range of selenium is 10 ~ 150mg/kg, which promotes the production and secretion of extracellular polysaccharide to some extent, indicating that Grifola frondosa has strong tolerance and enrichment ability to selenium, but the research on selenium-enriched fruiting bodies has not been reported.

2. The cultivated Grifola frondosa polysaccharide is the main component of its medicinal value. It is of great significance to improve the medicinal value of Grifola frondosa if the fruiting body or mycelium with high polysaccharide content can be cultivated. The research shows that different medium components have a very significant effect on the yield of Grifola frondosa polysaccharide. According to the research of Feng, Yao Kangke and others, the initial pH value of carbon and nitrogen and the dosage of soybean oil and potassium dihydrogen phosphate will affect the yield of polysaccharide from Grifola frondosa in liquid culture. Among them, carbon source and nitrogen source are the main factors affecting the formation of polysaccharide, and the best carbon source is glucose 3%+ potato 20%. When glucose is used as carbon source alone, the metabolism is fast, the acid production is early, the pH drops early, and the yield of polysaccharide is low. However, potato contains starch, which is continuously decomposed into glucose to supplement the carbon source and provide energy, which is more conducive to the production of polysaccharide. The best nitrogen source is organic nitrogen such as peptone, and the suitable carbon sources for polysaccharide formation are glucose 3%+ potato 20% starch 4%, glucose 2%+ sucrose 2%; The nitrogen source is peptone 0.6%, and the initial pH is 6.0. The optimum medium for sugar production is glucose 3%, potato 20%, protein 0.6%, soybean oil 0. 1%, magnesium sulfate 0.05% and potassium dihydrogen phosphate 0.05%. After 0/4 days of liquid culture/kloc-,the highest yield of polysaccharide was 34.65438+. It is feasible to improve the fermentation process and increase the yield of polysaccharide. In the production of Grifola frondosa fruiting body, the content of polysaccharide should be improved by improving the selection process of raw materials for strain breeding and improving the medical and health care function of Grifola frondosa.